Abstract
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
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CITATION STYLE
Guimaraes-Peres, A., Portaels, F., De Rijk, P., Fissette, K., Pattyn, S. R., Van Vooren, J. P., & Fonteyne, P. A. (1999). Comparison of two PCRs for detection of Mycobacterium ulcerans. Journal of Clinical Microbiology, 37(1), 206–208. https://doi.org/10.1128/jcm.37.1.206-208.1999
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