Non-coordinate regulation of endogenous epithelial sodium channel (ENaC) subunit expression at the apical membrane of A6 cells in response to various transporting conditions

Abstract

In many epithelial tissues in the body (e.g. kidney distal nephron, colon, airways) the rate of Na+ reabsorption is governed by the activity of the epithelial Na+ channel (ENaC). ENaC activity in turn is regulated by a number of factors including hormones, physiological conditions, and other ion channels. To begin to understand the mechanisms by which ENaC is regulated, we have examined the trafficking and turnover of ENaC subunits in A6 cells, a polarized, hormonally responsive Xenopus kidney cell line. As previously observed by others, the half-life of newly synthesized ENaC subunits was universally short (∼2 h). However, the half-lives of α- and γ-ENaC subunits that reached the apical cell surface were considerably longer (t1/2 > 24 h), whereas intriguingly, the half-life of cell surface β-ENaC was only approximately 6 h. We then examined the effects of various modulators of sodium transport on cell surface levels of individual ENaC subunits. Up-regulation of ENaC-mediated sodium conductance by overnight treatment with aldosterone or by short term incubation with vasopressin dramatically increased cell surface levels of β-ENaC without affecting α- or γ-ENaC levels. Conversely, treatment with brefeldin A selectively decreased the amount of β-ENaC at the apical membrane. Short term treatment with aldosterone or insulin had no effect on cell surface amounts of any subunits. Subcellular fractionation revealed a selective loss of β-ENaC from early endosomal pools in response to vasopressin. Our data suggest the possibility that trafficking and turnover of individual ENaC subunits at the apical membrane of A6 cells is non-coordinately regulated. The selective trafficking of β-ENaC may provide a mechanism for regulating sodium conductance in response to physiological stimuli.