Description of Gill Histopathology and Water Quality of Koi Fish (Cyprinus Koi fishio) Infected by Koi Herpes Virus (KHV)

Abstract

One of disease that attacks in common koi fish until now is caused by infection of Koi Herpes Virus. Koi Herpes Virus (KHV) is one type of DNA virus that attacks the family of Cyprinidae. Based on the preliminary test using PCR techniques gills, of koi fish infected by KHV was positive. This study aimed to determine water quality analysis and histopathological gills of koi fish (Cyprinus Koi fishio Linn) that infected KHV. Water quality measurements and gills tissue of fish observation was conducted in three repetition. Histopathological observations of gills tissue used for PCR analysis. The results of PCR analysis showed light line DNA band with size of 292 bp. The results of temperature, brightness, pH, DO, CO2 and COD are still in accordance with water quality standards, while values of ammonia showed 0.373-0.377 mg/l and BOD 3.986-4.729 mg/l that exceeding prescribed quality standards. Histopathological observations of gills tissue showed damage to the primary lamella form like edema, hyperplasia, atrophy, congestion, and necrosis than the type damage of secondary lamellae like edema, hyperplasia, fusion lamella, telangiectasis, congestion, and necrosis. Suspected KHV infection caused damage for gills of koi fish in histopathological.