CRISPR-Cas9 technology: a breakthrough in cancer gene therapy

Abstract

Gene editing, especially by clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9), has propelled gene function science. The rapid pace of gene editing has increased its medical/clinical significance CRISPR-Cas9, being very specific and potent, can screen the entire genome precisely and efficiently. Scientists have been able to manipulate genomes through crispr-cas9 systems in order to study tumor initiation, progression, and metastasis. Cancer therapy approaches have also heavily utilized this technology in recent studies. This is mainly because it has provided a powerful precision technology to manipulate the genome, thereby completely revolutionizing cancer research. The first crucial experimental application involved basic cancer gene functionality confirmation by a knockout technique in cell culture systems, specifically aimed at oncogenes. Subsequently, it enabled the incorporation of particular mutations into the DNA of study subjects, enabling the development of more precise models of tumors in live creatures. The current review is on crispr-cas9 in tumor therapy studies, which will have practical uses in acting as references for ongoing research on the pathogenesis and treatment of malignancy.