Bacillus cereus, a potential pathogen of snakehead fish Ophiocephalus argus

Abstract

Bacillus cereus is an emerging pathogen that has caused high mortalities in aquaculture animals. Yet the pathogenicity of B. cereus in snakehead fish Ophiocephalus argus is still unclear. In this study, a virulent strain (CA4) was isolated from diseased snakehead fish suffering from a typical symptom of hepatic hemorrhage with blood vessel congestion and macrophage infiltration, and was identified molecularly and phenotypically as B. cereus. It was β-hemolytic, showed an LD50 value of 2.57×106 CFU mL-1 for snakehead fish, and developed multiple resistances to cotrimoxazole, doxycycline, florfenicol, neomycin, sulfisoxazole, and tetracycline in aquaculture use. To the best of our knowledge, this is the first report of snakehead fish-pathogenic B. cereus. The findings of this study provide new insights into the potential threat of pathogenic B. cereus to snakehead fish.