Apical GLUT2 and Cav1.3: Regulation of rat intestinal glucose and calcium absorption

Abstract

We have proposed a model of intestinal glucose absorption in which transport by SGLT1 induces rapid insertion and activation of GLUT2 in the apical membrane by a PKC βII-dependent mechanism. Since PKC βII requires Ca2+ and glucose is depolarizing, we have investigated whether glucose absorption is regulated by the entry of dietary Ca2+ through Cav1.3 in the apical membrane. When rat jejunum was perfused with 75 mM glucose, Ca2+-deplete conditions, or perfusion with the L-type antagonists nifedipine and verapamil strongly diminished the phloretin-sensitive apical GLUT2, but not the phloretin-insensitive SGLT1 component of glucose absorption. Western blotting showed that in each case there was a significant decrease in apical GLUT2 level, but no change in SGLT1 level. Inhibition of apical GLUT2 absorption coincided with inhibition of unidirectional 45Ca2+ entry by nifedipine and verapamil. At 10 mM luminal Ca2+, 45 Ca2+ absorption in the presence of 75 mM glucose was 2- to 3-fold that in the presence of 75 mM mannitol. The glucose-induced component was SGLT1-dependent and nifedipine-sensitive. RT-PCR revealed the presence of Cavβ3 in jejunal mucosa; Western blotting and immunocytochemistry localized Cavβ3 to the apical membrane, together with Cav1.3. We conclude that in times of dietary sufficiency Cav1.3 may mediate a significant pathway of glucose-stimulated Ca2+ entry into the body and that luminal supply of Ca2+ is necessary for GLUT2-mediated glucose absorption. The integration of glucose and Ca2+ absorption represents a complex nutrient-sensing system, which allows both absorptive pathways to be regulated rapidly and precisely to match dietary intake. © 2007 The Authors. Journal compilation © 2007 The Physiological Society.