Construction of a High-density AFLP and SSR Map Using Recombinant Inbred Lines of Cultivated x Weedy Soybean

Abstract

A genetic linkage map of soybean was constructed by using a cross between a cultivar 'Keburi' and a weedy form of soybean 'Masshokutou Kou 502'. The parents differed in their ability in somatic embryogenesis. The mapping population consisted of 117 recombinant inbred lines (F11), and generated 30 linkage groups, which covered 2089 cM, including 515 amplified-fragment-length polymorphism (AFLP) markers and 85 simple sequence repeat (SSR) markers. For AFLP analysis, a simple technique using small polyacrylamide gels combined with silver staining was applied, which enabled a single person to construct a map in a short time at a reasonable cost. Comparison of this map with previously published maps revealed that 1) the level of polymorphism was the same as in a cultivar x cultivar cross; and 2) the cultivar x weed map was 13% shorter than the cultivar x wild map. The high-density map constructed in this study would contribute to the molecular mapping of quantitatively and qualitatively inherited characters from weedy forms of soybean.