Detection of Striped Jack Nervous Necrosis Virus(SJNNV) by Enzyme-Linked Immunosorbent Assay(ELISA)

Abstract

The present paper describes the detection of striped jack nervous necrosis virus (SJNNV), the causative agent of viral nervous necrosis, in eggs, larvae, and brood stocks of hatchery-reared and captured striped jack Pseudocaranx dentex by indirect ELISA. An appropriate condition for the ELISA was determined using purified SJNNV and the detection limit of virus antigens was ap proximately 5 ng/well. All samples of larvae supposed to be affected with the disease were proved positive for the presence of SJNNV antigens in this ELISA test. The viral antigens were also detected in some samples of fertilized eggs and ovaries of spawners but not in the testis or brain. Thus, it was indicated that spawners were an inoculum source of this viral disease in striped jack. © 1992, The Japanese Society of Fish Pathology. All rights reserved.