Thidiazuron- and zeatin-induced high-frequency shoot regeneration from leafand shoot-tip explants of strawberry

Abstract

Strawberry (Fragaria ×ananassa cv. Camarosa) was evaluated to determine a high-frequency shoot regeneration response for leaf and shoot-tip explants. For direct organogenesis fromstrawberry leaves, combinations of moderate concentrations of thidiazuron [TDZ (0, 2, and 4 μM)] and 6-benzylaminopurine [BAP (0, 4, and 9 μM)] added into medium containing Murashige and Skoog (MS) basal salts were compared. The most shoots regenerated per leaf explant were observed with 4-μM TDZ. Regeneration from shoot tips was evaluated with 0-, 2-, 4-, 8-, and 16-μM zeatin, kinetin, or 6-a,a-dimethylallylamino purine (2ip) tested individually. Optimum shoot proliferation was achieved from shoot-tip explants on medium containing 4-μM zeatin. Rooting was best without cytokinins in the medium; however, adequate rooting was obtained on the 4-μM zeatin treatment as well.