Effects of modification of amino groups with poly(Ethylene Glycol) on a recombinant uricase from Bacillus fastidiosus

Abstract

After modification with monomethoxyl-poly(ethylene glyeol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with mono- met ho\v I-polv( ethylene glycol).