HEPATOPROTECTIVE EFFECT OF POLYHERBAL FORMULATIONS ON PARACETAMOL INDUCED LIVER TOXICITY IN RATS

Abstract

The study was carried out to evaluate the hepatoprotective effect of polyherbal formulation YAK samples on paracetamol induced liver toxicity in rats. Albino rats were divided into six groups consisting of twelve animals in each group. Each group was subdivided into preventive & curative groups. Group I served as Healthy control. Group II received paracetamol (2 gm/kg, p.o. on 13th day), Groups III, IV, V and VI received Silymarin, YAK-001, YAK-PVX002 and YAK-PVZ003 respectively orally for 15 days. On 13th day 6 animals in each group were administered paracetamol (2 gm/kg, p.o) After 48 hours of paracetamol administration, these rats were subjected for preventive effect evaluation. Similarly, the animals in all the curative groups received a single dose of paracetamol (2 gm/kg, p.o.) on the 1st day followed by the respective drug treatment as in preventive group for 15 days to evaluate the curative effect. Serum biomarker levels were assessed in serum and histopathology of liver was assessed. Significant (p<0.01) rise in serum levels of SGOT, SGPT, ALP, GGT and ACP was observed in paracetamol (2 gm/kg, p.o.) treated rats. In contrast, treatment with YAK-001, YAK-PVX002 and YAK-PVZ003 exhibited the ability to counteract the paracetamol induced hepatotoxicity by decreasing the serum enzyme levels when compared to control in preventive and curative studies. YAK-001 showed enhanced preventive and curative effect in hepatotoxicity induced in rats than YAK-PVX002 and YAK-PVZ003 in albino rats. However, curative effect is enhanced hepatoprotection than preventive effect.