Efficacy of the entomopathogenic nematodes and their based-product on some species of mealybugs (Hemiptera: Pseudococcidae) in Egypt

Abstract

The infectivity of three Egyptian Heterorhabditid nematode species Heteror-habditis bacteriophora (Poinar), Heterorhabditis indica (Poinar) and Heterorhabditis baujardi (Poinar) (Rhabditida : Heterorhabdilidae) and one imported Steinernematid species, Steinernema abbasi (Elawad) (Rhabditida: Steinernematidea) were evaluated against preadult of the citrus mealybug Planococcus citri (Risso) (Hemiptera: Pseu-dococcidae) and adult of Egyptian mealybug Icerya aegyptiaca (Douglas) (Hemipte-ra: Monophlebidae) under laboratory conditions. Also, laboratory and semifield evaluation of the efficiency of entomopathogenic nematodes (EPN) alone or mixed with wax remover Oleyl-polypeptide, against adults and nymphs of the mealybug Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) were carried out. Nematode reproduction in their cadavers were detected. Moreover, the effect of Steinernema carpo-capsae alone or with medical additive (Docusate Sodium) comparing with Super Mi-srona oil were studied on the grape mealybug, Planococcus ficus (Signoret). Three methods of formulation of Egyptian entomopathogenic nematodes were tested for their effectiveness on H. bacteriophora and S. carpocapsae. These formulations are water-dispersible granules (WDG), Calcium alginate geland infected cadavers. The infectivity of the formulated nematodes was tested against I. aegyptiaca adults.