The Effects of Different DMA Level on Sperm Quality of Bangkok Chicken Offspring

Abstract

The objectives of this study were to determine the best DMA level to keep the quality of frozen sperm chickens in order to determine of freezing ability and the viability of spermatozoa in the female reproductive tract. Sperm was collected from 4 roosters offspring Bangkok chicken aged one years old. Sperm from 4 roosters was collected once a week and cryopreserved 24h in liquid nitrogen containers-196°C with Dimethylacetamide (DMA) concentration 3% (P1), 5% (P2), 10% (P3), 14% (P4), and 18% (P5). Frozen thawed sperm quality was observed after thawing at 4°C 60s. The results showed that freezing decreased motility, viability, and increase abnormalities. Different DMA concentration affected motility, viability, and abnormalities of frozen thawed sperm (P≤0.01). Motility (%) sperm after thawing P1, P2, P3, P4, P5 were 38,00±9.08, 46.00±8.94, 16.00±5.48, 8.00±2.24, and 1.00±2.74 respectively. Viability (%) sperm after thawing P1, P2, P3, P4, P5 were 40.10±7.21, 53.50±12.53, 22.00±4.43, 15.30±11.40, and 12.00±3.98 respectively. Abnormalities (%) sperm after thawing P1, P2, P3, P4, P5 were 60.40±7.40, 41.00±3.32, 67.20±4.09, 68.20±8.58, and 71.00±11.64 respectively. It can be concluded that the best DMA concentration for chicken sperm cryopreserved was 5% (P2).