lncRNA KHPS1 Activates a Poised Enhancer by Triplex-Dependent Recruitment of Epigenomic Regulators

Abstract

Blank-Giwojna et al. demonstrate that the antisense RNA KHPS1 forms an RNA-DNA triplex at the SPHK1 enhancer. Tethering KHPS1 to the enhancer is required for recruitment of E2F1 and p300, transcription of enhancer-derived RNA, and activation of SPHK1 expression. The results uncover a triplex-driven feedforward mechanism of transcriptional regulation.