Antibacterial Effect of Aqueous Extract of Theobroma Cacao against Gram-Negative and Gram-Positive Bacteria: An in Vitro Study

Abstract

Theobroma Cacao has a wide range against a range of diseases and an antimicrobial activity toward various pathogenic microorganisms which estimated of medicinal properties for the treatment of many infectious diseases. The aim of the research is to clarify the inhibitory action of Theobroma Cacao extract against human pathogenic bacteria also show the mechanism action of extract against biofilm formation and adherence of bacteria. The antimicrobial activity of the aqueous extract was tested based on agar-well diffusion assay and agar-disc diffusion, and this activity was compared with the antibiotic and measured which is more efficient. Furthermore, biofilm formation and adherence tests were done. Theobroma cacao (30%) was appeared in this research as high efficient against the wide spectrum of clinic isolates gram-negative and gram-positive bacteria (K.pneumonia, P.merabilis, P.vulgaris, S.typhimurum, S.typhi,, Staph. aureus, Staph.epidermidis, Staph.saprophytics and Strep.Pyogenes) which indicates that it is more effective than commercially available antibiotic in addition to a high inhibition against adherents and biofilm formation.