Effect of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in Kundhi buffalo bull and in vivo fertility rate

Abstract

Present study was conducted to determine the effect of in vitro selenium addition to the semen extender on chilled and frozen thawed quality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination, pH was determined by digital pH meter. Motility was determined under microscope at10x magnification, morphology and live dead ratio was assessed using eosin nigrosin stain under 40x magnification, concentration was calculated by hemocytometer, membrane integrity was examined by HOST solution. Conception rate was determined by inseminating 20 buffaloes (10 control and 10 best treatment group). After initial examination semen sample having motility ≥ 70% and morphology live dead ratio ≥ 80 % were diluted in Tris based extender consist of 0,2,4 and 6mM concentration of selenium. Four hours after dilution chilled semen was examined for post chilled semen quality, motility% (74.75±1.9), morphology% (86.62±1.2), membrane integrity% (87.87±0.6), live dead ratio% (87.62±0.7). After that samples were frozen for 24 hours, frozen thawed results showed motility (57.00±0.5), morphology (73.50±1.5), membrane integrity (70.00±0.01), live dead ratio (72.00±0.8 were high in 2mM/ml concentration of selenium. Overall results showed that 2mM/ml concentration of selenium improved chilled and frozen thawed quality of semen than control and other treatment groups. In conclusion, supplementation of selenium in Tris extender improved the chilled and frozen thawed quality characteristics and conception rate in Kundhi Buffalo bull semen.