Dry transportation of Oryzias wolasi embryo for ecotoxicological studies

Abstract

Fish of the genus Oryzias have long been used as model fish or sentinel organisms in ecotoxicological studies. Intercalibration between laboratories is fundamental in producing test procedures in ecotoxicological studies. In the inter-calibration activities, the transport of materials used in testing is important. Research on the dry transport of embryos, Oryzias wolasi was conducted. The test was carried out using a 24 well microplate. The parameters analyzed were the incubation period, hatching success, and the total length of fish at hatching. The experimental results showed that there were no statistically significant differences (p> 0.05) in the incubation and hatching success of the embryos were incubated in embryo rearing media (ERM) and those were incubated with dry incubation. However, there were significant differences (p> 0.05) in the total length of newly hatched larvae. The average of the larvae total length that were reared dry was longer compared to those were reared in ERM. The study suggested that the fish embryos of Oryzias wolasi could be transported dry for ecological studies and others.