Effects of splenectomy on spontaneously chronic pancreatitis in aly/aly mice

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Abstract

Background and Aim. Mice with alymphoplasia (aly/aly) mutation characterized by a lack of lymph nodes, Peyer's patches, and well-defined lymphoid follicles in the spleen were found. In this study, we used splenectomized aly/aly mice to elucidate the effects of secondary lymphoid organs in the development of aly/aly autoimmune pancreatitis. Methods. Forty-eight 10-week-old aly/aly mice were divided into two groups for splenectomy and sham operation. Histological and immunohistochemical analyses of the pancreas were performed at the ages of 20, 30, and 40 weeks old after operation, respectively. Results. Our results showed that mononuclear cell infiltration was restricted to the interlobular connective tissues at the age of 20 weeks, and not increase obviously at the age of 30 and 40 weeks in splenectomized aly/aly mice. Furthermore, an apparent decrease in the expressions of CD 4 + T, CD 8+ T, and B cells was detected in the pancreatic tissues compared with sham aly/aly mice, however, no significant difference in macrophage expression between mice with and without a splenectomy. Conclusions. Inflammation infiltration and development of the pancreatitis in aly/aly mice were suppressed effectively after splenectomy, which was, at least partly, correlated to inhibition of the infiltration of T and B cells in pancreatic tissues but not to macrophages. © 2010 Heng-XiaoWang et al.

Figures

  • Figure 1: Chronic inflammatory changes of splenectomized (Sp) and sham aly/aly mice at the ages of 20, 30, and 40 weeks old, respectively. The pancreas was histopathologically examined (refer to Figure 2). Grading of inflammatory lesions of the pancreas: 0, no visible change; (1), focal accumulation of lymphoid cells; (2), focal accumulation of lymphoid cells with evidence of tissue damage; (3), extensive lymphoid cell infiltration with tissue damage (<30%); (4), replacement by adipose tissue (>30%).
  • Figure 2: Histopathological analysis of effects of splenectomy (sp) on pancreatitis developing in aly/aly mice at the ages of 20, 30, and 40 weeks old, respectively. Sham 20 w, 30 w, and 40 w indicated sham-group mice at the ages of 20, 30, and 40 weeks old, respectively. Arrows, indicating lymphoid cell infiltration and ∗indicating some pancreatic ducts of proliferation and regeneration. a, artery; d, pancreatic duct; La, islet of Langerhans; v, vein. Scale bars: 200 μm.
  • Figure 3: Immunohistopathological analysis of pancreas in splenectomized (Sp) and sham aly/aly mice by CD4. Arrows, indicating CD4positive cells. a, artery; d, pancreatic duct; La, islet of Langerhans; v, vein. Scale bars: 100 μm (c); 200 μm ((a), (b), (d), (e), and (f)).
  • Figure 4: Immunohistopathological analysis of pancreas in splenectomized (Sp) and sham aly/aly mice by CD8a, B220, and F4/80. Arrows, indicating CD8- ((a) and (d)), B220- (e), and F4/80- ((c) and (f)) positive cells. a, artery; d, pancreatic duct; La, islet of Langerhans; v, vein. Scale bars: 100 μm ((a), (b), and (d)); 200 μm ((c), (e), and (f)).
  • Figure 5: Immunostaining positive scoring of the pancreas was evaluated as a percentage of the total area of the specimen in splenectomized (Sp) and sham aly/aly mice at the ages of 20, 30, and 40 weeks old, respectively. Results are expressed as the mean ± SEM. ∗P < .05; ∗∗P < .01.

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CITATION STYLE

APA

Yi, S. Q., Wang, H. X., Li, J., Terayama, H., Naito, M., Hirai, S., … Itoh, M. (2010). Effects of splenectomy on spontaneously chronic pancreatitis in aly/aly mice. Clinical and Developmental Immunology, 2010. https://doi.org/10.1155/2010/614890

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