Non-denaturing purification of alpha-Synuclein from erythrocytes

Abstract

Pathogenic aggregation of α-Synuclein (αSyn) is implicated in familial and sporadic Parkinson disease (PD) and several other synucleinopathies. Choosing non-denaturing conditions, we developed a several purification schemes in order to obtain αSyn from human erythrocytes, enabling researchers to employ biophysical techniques in order to elucidate its native structure and properties.