Diffuse reflection electron spectroscopy in study of blood serum with multiple myeloma

Abstract

Subject of Research. The paper presents testing and evaluation of the spectral method potential capabilities in relation to the study of human blood serum in normal and in patients with multiple myeloma. The study of samples of native and lyophilized serum is carried out in comparison with the spectral characteristics of aqueous extracts of animal muscle sarcoplasm. Method. A non-destructive diffuse reflection electron spectroscopy method with a vertical beam path in the wavelength range of 200–700 nm in the absorption format was used. Main Results. In contrast to the native donor serum, a band of mucopolysaccharides (410–415 nm) of different intensity has been observed in the spectra of samples of animal tissue sarcoplasm extracts. This band appeared in the spectra of donor serum after lyophilization. That is why the presence of the band of mucopolysaccharides in the patients’ serum spectra is associated with reduced moisture binding capacity of the protein-polysaccharide complex, which, in turn, is due to the features of protein secondary structure in different forms of multiple myeloma. Practical Relevance. The peculiarities in the similarities and differences between blood serum spectra of donors and patients give reason to believe that the method of diffuse reflection electron spectroscopy, that does not require a lot of material and preliminary sample preparation, can be promising as a rapid tool for additional initial diagnosis and the treatment process monitoring of multiple myeloma and other lymphoproliferative diseases.