Contrasting changes of photosystem 2 efficiency in Arabidopsis xanthophyll mutants at room or low temperature under high irradiance stress

Abstract

We compared the responses of wild type (WT) and three mutants including npq1 (lutein-replete and violaxanthin deepoxidase-deficient), lut2 (lutein-deficient), and lut2-npq1 (double mutant) to high irradiance (HI, 2 000 μmol m-2 s-1) at both low (LT, 5°C) and room (25°C) temperature. Xanthophyll-dependent energy dissipation was highest in the WT, followed by the lut2, npq1, and npq1-lut2. At 25°C the relative stress tolerance expressed by Fv/Fm was consistent with the energy dissipation capacity for the first 2 h of treatment. After 3-4 h, the Fv/Fm levels in lut2 and npq1 converged. Under combined LT and HI the relative tolerance sequence was in contrast to the energy dissipation capacity being WT 〉 npq1 〉 lut2 〉 lut2-npq1. There were little or no significant change in the contents of xanthophylls and carotenes or the chlorophyll (Chl) a/b ratio in any of the materials. Thus lutein (L) substitution possibly alters the conformation/organisation of L binding proteins to enhance damage susceptibility under HI at LT. The enhanced vulnerability is not compensated for the energy dissipation capacity in the lut2 background at LT.