Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm

Abstract

The objective of this study was to analyze the effectiveness of Propolis Fluoride in inhibiting the biofilm formation of S. mutans, S. gordonii and S. sanguinis in comparison to Silver Diamine Fluoride. Bacteria suspensions were cultured in Brain Heart Infusion Broth, then dripped onto a 96-well plate. Propolis fluoride was added to the well plates, which were then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. Afterwards, Crystal Violet 0.5% was added to each well plate, and a micro plate reader was used to evaluate the Optical Density value at 570nm wavelength. S. gordonii and S. mutans were analyzed using MTT assay by inserting the bacterial suspensions into a BHI medium enriched with 0.2% sucrose and exposed to PPF, then incubated at 4 different times. The ANOVA test result on the potential of Propolis Fluoride to inhibit biofilm formation of S. mutans, S. gordonii and S. sanguinis was equal to 38%.(b/b) Silver Diamine Fluoride, Propolis Fluoride has the same inhibition potential and effectiveness against biofilm formation as Silver Diamine Fluoride.