Characteristics of lipid components and triacylglycerol molecular species of jack bean (canavalia gladiata DC.)

Abstract

Lipids extracted from two cultivars of Jack bean (Canavalia gladiata DC.) were classified by thin-layer chromatography (TLC) into nine subfractions. The major lipid components were triacylglycerols (TAG: 43.8 - 45.7%) and phospholipids (PL: 46.7 - 47.0%). Other lipids were present at a concentration of 0.3 - 2.7%. The molecular species and FA distributions of TAG, isolated from the total lipids in the beans, were analyzed by a combination of AgNO 3-TLC and gas chromatography (GC). Eighteen TAG molecular species were detected in the beans. The dominant TAG species were M3 (32.5 - 33.0%), followed by S2M (5.4 - 5.5%), SM2 (7.6 - 7.7%), S2D (9.0 - 9.1%), SMD (5.3 - 5.4%), S2T (6.3 - 6.4%), M2D (5.3 - 5.6%), MD2 (6.5 - 6.7%), SDT (5.2 - 5.3%) and M2T (3.8 - 3.9%), where S, M, D, and T denote saturated FA, monoene, diene, and triene, respectively. The results show that the lipid classes, FA distributions and TAG molecular species of Jack bean are not dependent on the cultivar.