Detection of duck enteritis virus by polymerase chain reaction

Abstract

Duck enteritis virus (DEV), a herpesvirus, is the causative agent of duck viral enteritis in free-flying, feral, and domesticated members of the Anatidae family. HindIII-digested DEV DNA was cloned into the plasmid pBluescript, and a 1.95-kb fragment was sequenced. This fragment codes for the 3' region of the DEV homologues of varicella-zoster virus (VZV) open reading frame (ORF) UL6 and the 5' region of VZV UL7. Alignment of the putative peptide fragments for DEV UL6 and UL7 showed a 64% and 37% identity with VZV UL6 and UL7, respectively. Primers located in the highly conserved domain of the UL6 gene were used for a polymerase chain reaction (PCR) assay, which was able to amplify DEV DNA. The PCR assay also amplified DEV DNA from the original outbreak samples and/or after passage in Muscovy duck embryos.