HORIZONTAL TRANSFER OF PLASMID DNA BETWEEN DIFFERENT BACTERIA SPECIES UNDER MICROBIAL INTERACTIONS

Abstract

Horizontal transfer of plasmid DNA was investigated under phytoplankton metabolites / zooplankton predation exposure condition, to obtain some basic information about the prosperity and decay of GMO (genetically modified microorganisms) in filed release was investigated in this study. Escherichia coli HB101, E. coli C600, E. coli S17-1, Pseudomonas aeruginosa PAO1 (gram-negative) and Bacillus cereus MC (gram-positive) as recipient strain of plasmid DNA, E. coli HB101/pBR325, E. coli C600/RP4 and E. coli S17-1/pSUP104 as donor of plasmid DNA, were supplied. As phytoplankton, Microcystis aeruginosa (cyanophyceae), Melosira varians (bacillariophyceae) and Scenedesmus quadricauda (chlorophyceae) collected from Lake Tega as donor of metabolites, were supplied. As zooplankton, Tetrahymena pyriformis (cilliata) and Philodina erythrophthalma (rotifer) collected from Lake Tega as predator were supplied. The results can be concluded as follows; 1) Phytoplankton metabolites leads acceleration of horizontal transfer between not only same strains but also different strain in spite of whether transmissible or not, 2) Zooplankton predation leads decrease of bacterial individual number and horizontal transfer of plasmid DNA, and 3) Horizontal plasmid DNA transfer is influenced greatly, because the natural ecosystem includes phytoplankton as producer and zooplankton as consumer in the same time.