Antifungal activity and gas chromatography coupled with mass spectrometry (GC-MS) leaf oil analysis of essential oils extracted from Eucalyptus globulus (Myrtaceae) of north centre region of Morocco

Abstract

Bovine mastitis is the inflammation of the parenchyma of the mammary glands of cattle associated with microbial infections. The most common bacteria causing bovine mastitis include; Staphylococcus aureus. Monolaurin, a food grade glycerol monoester of lauric acid, has been reported to have the greatest antimicrobial activity of all of the monoglycerides. The aim of this study was to evaluate of antibacterial effect of monolaurin on S. aureus isolated from bovine mastitis in Iran. Milk samples were aseptically collected from each quarter of 20 cows and after isolation and identification of S. aureus, 0.5 Macfarland of this bacteria were collected and added to BHI agar with different concentrations of moneulaurin (0, 5, 10, 100, 500, 1000 and 2000 ppm), then S. aureus colony count was done and compared to the control (0 ppm of monolaurin). By attention to results of this study monolaurin showed a concentration dependent antibacterial effect. Finally, results of this study demonstrated monolaurin has antimicrobial activity against S. aureus isolated from bovine mastitis.