Plant regeneration capacity of calluses derived from mature seed of five Indonesian rice genotypes

Abstract

Establishment of a suitable system for plant regeneration of rice calluses derived from mature seed is a prerequisite for genetic transformation using callus as the target tissue. Selecting the most suitable medium and assessing the genotype performance for in vitro response are essential requirement for this purpose. The experiment with five Indonesian rice genotypes showed that callus-proliferation capacity (CPC) and callus-growth capacity were significantly affected by genotype and CPC by medium. The shoot-regeneration capacity and plantlet-regeneration capacity were affected by the interaction effect between genotype and medium. However for green plant-regeneration capacity, it was affected independently by genotype and medium. Culture media D1 and NB 5 were the most suitable media for callus subculture and plant regeneration, respectively. Genotypes Fatmawati and BP-140 consistently performed best in the callus subculture and plant regeneration.