Effectiveness of Bdellovibrio Against the Immune System of White Shrimp (Litopenaues vannamei) Infeksion with Vibrio harveyi Bacteria

Abstract

White shrimp (Litopenaeus vannamei) is an aquatic biota that is included in the leading commodity of aquaculture. The Vibrio harveyi bacteria can cause up to 80% death in white shrimp. The predatory Bdellovibrio is highly regarded as an alternative source of antibiotics and has been reported to have the potential to control shrimp pathogens such as Vibrio chalerae and Vibrio parahaemolyticus. The aim of this study was to analyze the effect of giving Bdellovibrio on the immune system of vaname shrimp infected with Vibrio harveyi bacteria. This research is experimental using a completely randomized design (CRD), which consists of 5 treatments and 3 replications. This study was experimental using a completely randomized design (CRD), which consisted of 5 treatments and 3 replications. Treatments in the study included P1 (+)(Not given Bdellovibrio and tested with Vibrio harveyi), P2 (-) (Not given Bdellovibrio and tested with NaCL), P3 (Given 30 ppm Bdellovibrio and tested with Vibrio harveyi), P4 (Given Bdellovibrio 50 ppm and tested with Vibrio harveyi), and P5 (Given Bdellovibrio 70 ppm and tested with Vibrio harveyi). In this study, the best treatment value was obtained, namely P5 treatment, the THC value was 16.94 x 106 cells/ml, the DHC value in hyaline cells was 59.33%, the granular cells were 24%, the semi-granular cells were 16.67%, the AF value 64.82%, TBC value 5.98 x 108 CFU/ml, TVC value 1.43 x 107 CFU/ml, FCR value 1.23, specific length growth rate 19.4%/day, specific weight growth rate 9 .96%/day, the absolute length value is 8.73 cm, the absolute weight is 61 grams and the SR value is 77.78%. From this research, it was concluded that administration of Bdellovibrio in the rearing media at different doses was able to improve the immune system and survival of White shrimp tested with Vibrio harveyi bacteriaUdang putih (Litopenaeus vannamei) merupakan biota perairan yang termasukdalam komoditas unggulan budidaya perikanan. Bakteri Vibrio harveyi dapatmenyebabkan kematian pada udang vaname hingga 80%. Bdellovibrio predatorsangat dianggap sebagai sumber antibiotik alternatif dan dilaporkan berpotensimengendalikan patogen udang seperti Vibrio chalerae dan Vibrio parahaemolyticus.Tujuan penelitian ini adalah menganalisis pengaruh pemberian Bdellovibrio terhadapdaya tahan tubuh udang vaname yang terinfeksi bakteri Vibrio harveyi. Penelitian inibersifat eksperimental dengan menggunakan rancangan acak lengkap (RAL) yangterdiri dari 5 perlakuan dan 3 ulangan. Penelitian ini bersifat eksperimental denganmenggunakan rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 3ulangan. Perlakuan dalam penelitian meliputi P1(+)(Tidak diberikan Bdellovibrio dandiuji dengan Vibrio harveyi), P2 (-) (Tidak diberikan Bdellovibrio dan diuji denganNaCL), P3 (Diberikan Bdellovibrio 30 ppm dan diuji dengan Vibrio harveyi), P4(Diberikan Bdellovibrio 30 ppm dan diuji dengan Vibrio harveyi), P4 (DiberikanBdellovibrio 30 ppm dan diuji dengan Vibrio harveyi), P4 (Diberikan Bdellovibrio 50ppm dan diuji dengan Vibrio harveyi), dan P5 (Diberikan Bdellovibrio 70 ppm dandiuji dengan Vibrio harveyi). Pada penelitian ini diperoleh nilai perlakuan terbaikyaitu perlakuan P5, nilai THC sebesar 16,94 x 106 sel/ml, nilai DHC pada sel hialinsebesar 59,33%, sel granular 24%, sel semi granular 16,67%, nilai AF 64,82%, nilaiTBC 5,98 x 108 CFU/ml, nilai TVC 1,43 x 107 CFU/ml, nilai FCR 1,23, lajupertumbuhan panjang spesifik 19,4% /hari, laju pertumbuhan bobot spesifik 9,96%/hari, nilai panjang absolutnya adalah 8,73 cm, berat mutlak 61 gram dan nilaiSR 77,78%. Dari penelitian ini disimpulkan bahwa pemberian Bdellovibrio padamedia pemeliharaan dengan dosis berbeda mampu meningkatkan daya tahan tubuhdan kelangsungan hidup udang putih yang diuji dengan bakteri Vibrio harveyi.