Abstract
We describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with interstrand crosslinks. Crosslinked templates (xHDRTs) increase Cas9-mediated editing efficiencies by up to fivefold in K562, HEK293T, U2OS, iPS and primary T cells. Increased editing from xHDRTs is driven by events on the template molecule and requires ataxia telangiectasia and Rad3-related (ATR) kinase and components of the Fanconi anemia pathway.
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CITATION STYLE
Ghasemi, H. I., Bacal, J., Yoon, A. C., Tavasoli, K. U., Cruz, C., Vu, J. T., … Richardson, C. D. (2023). Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells. Nature Biotechnology, 41(10), 1398–1404. https://doi.org/10.1038/s41587-022-01654-y
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