The diagnostic value of immunohistochemistry in the typing of renal tumors with eosinophylic cytoplasma

Abstract

AIM: In this study, we aimed to review the diagnostic approach to eosinophilic cell renal neoplasms by light microscopy and immunohistochemical techniques. METHOD: In this study 23 of these tumors were eosinophilic variant classic RCC, 15 eosinophilic variant papillary RCC, 13 eosinophilic variant chromophobe RCC and 13 oncocytoma cases. These tumors were immunohistochemically treated with CK7, CD117, EpCAM, Vimentin, RCCm (Renal cell carcinoma marker) and GST-α. RESULTS: In our study, contrary to the general literature on Vimentin, 65.2 % negativity was found in our patients with eosinophilic variant classic RCC. However, when compared with other tumor types in our study, vimentin expression was highest in eosinophilic variant classical RCC with 34.8 %. Statistically; RCCm, GST-α, EpCAM, CD117, CK7 were found to be signifi cantly associated with tumor types, while no signifi cant relationship was found between Vimentin and tumor types. RCCm positivity and CK7 and CD117 negativity were in favour of eosinophilic variant classical RCC, EpCAM, CK7 and CD117 positivity and Vimentin, GST-α and RCCm negativity supported eosinophilic variant chromophobe RCC, CK7 and RCCm positivity and CD117 and GST-α negativity were found in favour of eosinophilic variant papillary RCC. CD117 positivity and Vimentin, CK7 and GST-α negativity were found to support oncocytoma. CONCLUSIONS: The panel with RCCm, GST-α, EpCAM, CD117, CK7 will contribute to the differentiation of eosinophilic cytoplasm renal tumors that cannot be determined by morphological fi ndings and to reach the correct diagnosis (Tab. 3, Fig. 4, Ref. 54).