A recombinant avian leukosis virus subgroup J for directly monitoring viral infection and the selection of neutralizing antibodies

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Abstract

Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test.

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Wang, Q., Li, X., Ji, X., Wang, J., Shen, N., Gao, Y., … Wang, X. (2014). A recombinant avian leukosis virus subgroup J for directly monitoring viral infection and the selection of neutralizing antibodies. PLoS ONE, 9(12). https://doi.org/10.1371/journal.pone.0115422

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