Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems

Abstract

Spectral luminescent analysis of single cells and cellular systems enable us to reveal the initial changes of intracellular metabolism that can followed by human diseases or failure in biocenosis. Two cytodiagnostic systems of devices and techniques have been developed: 1) Microspectrofluorimeters registering the fluorescent spectra of individual cells or intracellu-lar organelles used for fundamental investigations of cell reactions and for discovering and studying new dimensionless fluorescent characteristic parameters reflecting the biochemical or physiological properties of the cells; 2) Double and multi-wave microfluorimeters for rapid registration of fluorescent characteristic parameters for many cells to obtain statistical information about cell population. These techniques are useful especially in medical and ecological investigations.