The use of PCR and real-time PCR for qualitative and quantitative determination of poultry and chicken meals

Abstract

European Union regulations (e.g. Commission Regulation (EU) No 56/2013) set restrictions on the use of poultry meals in animal nutrition, which requires the species composition of manufactured feeds to be constantly monitored. The aim of this study is to develop a method for qualitative analysis of poultry meals, enabling the four main poultry species (chicken, Turkey, duck, goose) to be detected using one pair of primers, and a method for quantitative determination of chicken meals in poultry meals. The qualitative identification method was developed using PCR technology, whereas qPCR and TaqMan probes were used for quantitative identification. The study was performed with samples of feed mixture containing poultry and chicken meal. The limit of determination was 0.08% and 0.02% for qualitative and quantitative identification, respectively. The results of quantitative determinations obtained for independent DNA isolations from the same samples are repeatable (RSDcT ≤0.46%). The determined concentrations are accurate (Dc ≤11.23% for c ≥0.06). The identification of target sequences in both tests is good enough for commercial applications.