Development of immunocolloidal strip for rapid detection of pyrimethanil

Abstract

In this study, we prepared a monoclonal antibody against pyrimethanil, for which we first derived its hapten according to the structural formula of pyrimethanil; we used indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to screen positive mice for cell fusion; finally, we developed five cell lines (1A5, 1E7, 3A6, 3H12 and 5D4) that produced monoclonal antibodies to pyrimethanil. The monoclonal antibody produced by each cell was detected by ic-ELISA, and the monoclonal antibody produced by the 1E7 cell strain was found to be the most sensitive to pyrimethanil. The IC50 value of this antibody was 4 ng/ml. After using this monoclonal antibody, i.e. 1E7, to perform immunocolloidal gold strip treatment on cucumber samples, the PBS and the cucumber samples cutoff value of Immunochromatographic strips (ICS) was found to be 50 ng/ml; We conclude that ICS can rapidly detect pyrimethanil in cucumber samples.