Esterase-Mediated Highly Fluorescent Gold Nanoclusters and Their Use in Ultrasensitive Detection of Mercury: Synthetic and Mechanistic Aspects

Abstract

The fast, accurate, and ultrasensitive detection of toxic mercury in real water samples is still challenging without the use of expensive sophisticated instruments. Herein, highly fluorescent gold nanoclusters (AuNCs) were synthesized using a newer protein templet, esterase (EST). The EST-AuNCs consisted of ∼25 Au atoms in the nanocluster having ∼2 nm size. EST-AuNCs were found to be highly stable in aqueous buffer with a wide range of pH (pH 4-12) and were also stable in powdered form. The fluorescence quantum yield of EST-AuNCs in deionized water was 6.2% which had increased to 7.8% upon the addition of 1 M NaCl (an increase of 23%). The EST-AuNCs selectively sense the toxic Hg2+ ions with higher sensitivity (limit of detection; 0.88 nM) with the linear range 1-30 nM. The test strips for rapid sensing of Hg2+ in real water samples were developed on the polymeric surface. The validation of sensing ability of EST-AuNCs suggested 94-98% recovery with linearity. Moreover, because of the widely reported applications of EST, the developed EST-AuNCs could also be used for another sensing, catalytic, and biomedical applications.