Transporte de oócitos bovinos em meio de maturaccedil;ão por diferentes períodos de tempo sem controle da atmosfera gasosa

Abstract

The viability of the transport of the bovine oocytes was evaluated in chemically defined medium and the need for the addition or not of hormones in this medium was analyzed. The oocytes in the control group (0h) were matured for 24h in CO2 incubator, and in experimental groups they were transported in portable incubator. In experiment I, the cleavage rates were similar (P>0.05) to the groups 0h (59.7%), 3h (53.5%), and 9h (48.8%), but they decreased in groups 6h (46.1%) and 12h (43.8%), however, these rates were similar among the groups 3h, 6h, 9h, and 12h. The production of blastocysts was not different (P>0.05) for groups 0h (38.0%), 3h (32.3%), 6h (27.3%), and 9h (24.8%), but there was a reduction in the 12h group (18.9%). These rates were similar among the groups 6h, 9h and 12h. In experiment II, no significant difference (P>0.05) was observed among the rates of cleavage for the groups 0h (71.4%), 3h with (70.3%) and without hormones (64.8%), and 6h with (56.0%) and without hormones (54.1%). The production of blastocysts was similar (P>0.05) for groups 0h (46.1%) and 3h with (45.8%) and without hormones (41.1%), but decreased in groups 6h with (35.5%) and without hormones (33.5%). These rates were similar among the groups 3h without, 6h with and without hormones. These results indicate the possibility of the transport of bovine oocytes up to 9h, and the addition of hormones in this medium does not influence the rates of cleavage and blastocysts.