Nervous necrosis virus (NNV) -susceptibility and -productivity of newly established cell lines derived from sevenband grouper hyporthodus septemfasciatus

Abstract

―Multiple cell lines permissive to nervous necrosis virus (NNV) infection may be necessary for identifying receptor binding sites of viral surface protrusions. We have established new cell lines, SeGE-12, SeGE-22, SeGF and SeGB, derived from eyed-egg embryos, fin and brain of sevenband grouper. The optimum growth temperatures of these cell lines were all around 30°C. The growth temperature range for SeGE-12 and SeGE-22 was 25°C–32°C, whereas for SeGF and SeGB it was 15°C–32°C. These cell lines exhibited no cytopathic effect (CPE) due to NNV infection, but released extracellular NNV at 102.8 to 104.7 TCID50/mL after 14 days incubation.