Ultrasound assisted extraction for analysis of total phenolic content and antioxidant activity of medicinal plants

Abstract

In the present study, Bupleurum chinense, Isatis indigotica Fort., Kadsura coccinea, Notopterygium incisum and Rhizoma Anemarrhenae were investigated for total phenolic contents (TPC) in water, ethanol and methanol solvent. The antioxidant activities of exts. were detd. by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities (DPPH%) and reducing power (RP) assay. The results showed that at 1.0 mg/mL, the methanol ext. of R. Anemarrhenae has exhibited highest TPC, 9.36 mg GAE/g dw, followed by the ethanol ext., 8.14 mg GAE/g dw. Antioxidant activities were also analyzed. The highest DPPH scavenging capacity with the lowest IC50 value, 0.21 mg dw/mL was found in methanol ext. of R. Anemarrhenae. The reducing power value with 15.86 mg AAE/g in methanol ext. of R. Anemarrhenae was obsd. A high correlation was established between the RP value, DPPH% and the TPC. Consequently, R. Anemarrhenae could be selected as an excellent antioxidant for functional foods as well as pharmaceutics. [on SciFinder(R)]