Comparação das técnicas de captura de híbridos e PCR para a detecção de HPV em amostras clínicas

Abstract

Introduction and objective: More than 100 types of human papillomaviruses (HPV) are known, of which 30 have been reported in anogenital infections. The infection has clinical importance, inasmuch as some viral types are associated with lesions that can progress to cervical cancer. Molecular methods are considered an important tool for the diagnosis of this infection. The objective of this study was to compare the detection of high risk HPV using hybrid capture 2 with HPV detection by conventional and real time PCR. Methodology: 56 ectocervical samples were analyzed by hybrid capture and after that by conventional and real time PCR. Results: Both PCR and RT-PCR showed a high degree of correlation (95.1%), whereas the comparison between PCR and HC2 showed a fair correlation (90.2% and 87.8% for PCR and RT-PCR, respectively). Discussion and conclusions: HC is widely accepted for the detection of HPV, however, it may lack sensitivity in comparison with PCR techniques. RT-PCR has further advantages over the conventional PCR in terms of speed as well as it is slightly more sensitive. Due to their high sensitivity and fast response, PCR methods could be used as a screening method for HPV detection in ectocervical samples.