Perbandingan Antioksidan Ekstrak Etanol Daun Gaharu (Aqularia malaccensis L.) dan Ketiga Fraksi Berbagai Pelarut (Heksan, Etil Asetat, dan Air)

Abstract

Keyword: Antioxidant; Aquilaria malaccensis L.; DPPH (2,2-diphenyl picrylhydrazyl) Kata Kunci: Antioksidan; Aquilaria malacensis L.; DPPH (2,2-Difenil-1 Pikrilhidrazil) ABSTRACT: The research comparison of antioxidant activity extracts and various fractions of Aquilaria malaccensis L. leaves had been done. Extraction had been done by maceration method of 350 grams of leaf powder used ethanol 70% solvent for 3 days to 5 repetitions, then fractionation used hexane, ethyl acetate and akuades solvents. The extraction yield obtained 3.57 (%w/w) viscous extract yield, while the fractionation results obtained hexane, ethyl acetate and water fractions were 43.14, 32.67, and 22.20 (%w/w). Antioxidant test was carried out used DPPH (diphenyl-picrylhydrazyl) method, where the inhibitory value of DPPH (% inhibition) was tested at various concentrations of sample test solutions, for thick extracts at concentrations of 80; 60; 40; 20; and 10 ppm, and fraction samples at concentrations 200; 160; 120; 80; and 40 ppm. IC50 calculation results showed that antioxidant activity was classified as very strong in thick extracts and ethyl acetate fraction, were 16.45 and 47.69 ppm, and strong antioxidants were found in n-hexane fraction and water fraction were 57.24 and 76.95 ppm