DNA Sequence Plays a Major Role in Determining Nucleosome Positions in Yeast CUP1 Chromatin

33Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

The role of DNA sequence in determining nucleosome positions in vivo was investigated by comparing the positions adopted by nucleosomes reconstituted on a yeast plasmid in vitro using purified core histones with those in native chromatin containing the same DNA, described previously. Nucleosomes were reconstituted on a 2.5 kilobase pair DNA sequence containing the yeast TRP1ARS1 plasmid with CUP1 as an insert (TAC-DNA). Multiple, alternative, overlapping nucleosome positions were mapped on TAC-DNA. For the 58 positioned nucleosomes identified, the relative positioning strengths and the stabilities to salt and temperature were determined. These positions were, with a few exceptions, identical to those observed in native, remodeled TAC chromatin containing an activated CUP1 gene. Only some of these positions are utilized in native, unremodeled chromatin. These observations suggest that DNA sequence is likely to play a very important role in positioning nucleosomes in vivo. We suggest that events occurring in yeast CUP1 chromatin determine which positions are occupied in vivo and when they are occupied.

Cite

CITATION STYLE

APA

Shen, C. H., & Clark, D. J. (2001). DNA Sequence Plays a Major Role in Determining Nucleosome Positions in Yeast CUP1 Chromatin. Journal of Biological Chemistry, 276(37), 35209–35216. https://doi.org/10.1074/jbc.M104733200

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free