Chromatogram-bioactivity correlation-based discovery and identification of three bioactive compounds affecting endothelial function in ginkgo biloba extract

Abstract

Discovery and identification of three bioactive compounds affecting endothelial function in Ginkgo biloba Extract (GBE) based on chromatogram-bioactivity correlation analysis. Three portions were separated from GBE via D101 macroporous resin and then re-combined to prepare nine GBE samples. 21 compounds in GBE samples were identified through UFLC-DAD-Q-TOF-MS/MS. Correlation analysis between compounds differences and endothelin-1 (ET-1) in vivo in nine GBE samples was conducted. The analysis results indicated that three bioactive compounds had close relevance to ET-1: Kaempferol-3-O-α-L-glucoside, 3-O-2-O-6-O-[P-OH-trans-cinnamoyl]-β-D-glucosyl-α-rhamnosyl Quercetin isomers, and 3-O-2-O-{6-O-[P-OH-trans-cinnamoyl]-β-D-glucosyl-α-rhamnosyl Kaempferide. The discovery of bioactive compounds could provide references for the quality control and novel pharmaceuticals development of GRE. The present work proposes a feasible chromatogram-bioactivity correlation based approach to discover the compounds and define their bioactivities for the complex multi-component systems.