Immunolocalization of steroidogenic enzymes P450scc, 3βHSD, and P450c17 in the ovaries of wild raccoon dogs (Nyctereutes procyonoides)

Abstract

We investigated the distribution of 3 types of steroidogenic enzymes, P450scc, 3βHSD, and P450c17, in wild raccoon dog ovaries by immunohistochemistry. Six pairs of ovaries were obtained from wild raccoon dogs between 2001 and 2003, with 3 of the 6 pairs of ovaries containing corpora lutea. P450scc, 3βHSD, and P450c17 were localized in the granulosa and theca cells of these raccoon dogs. Furthermore, lutein cells were stained positively for P450scc and 3βHSD in the pregnant and non-pregnant raccoon dogs. These results suggest that granulosa and theca cells may synthesize progesterone and androgens, which may play an important role in follicular development, and that lutein cells are a major source of progesterone in wild raccoon dogs.