Role of Cystatin-C as Serum Biomarkers in Predicting Glomerular Function-Associated with Copper-Induced Acute Kidney Injury

Abstract

Cystatin C is a 13 kD molecular-weight protein synthesized by all nucleated cells which functions as a cysteine protease inhibitor. Cystatin C is detectable when kidney function decreases due to the excessive accumulation of nephrotoxic substances such as copper. Previous studies have proven that, white turmeric rhizome can act as a nephroprotector agent at a dose of 500 mg/BW. The purpose of this study is compare Cystatin-C marker and serum creatinine as biomarkers in the examination of acute kidney injuries induced by ncphrotoxic substance. This was a post-test only controlled experimental study on wistar strain male rats that were divided randomly using simple random sampling approach into three groups; normal control group, treatment control group (Curcumin for 2 weeks followed by CuSO4 for 3 days at each weekend), and CuSO4 pentahydrate control group. This research is conducted at Faculty of Pharmacy and Faculty of Medicine at the University of North Sumatera in May to August 2019. The analysis results is normally distributed and has significant differences in levels of Cystatin-C, creatinine and protein serum due to differences in the treatment of each group where p<0.05. Serum Cystatin-C as a biomarker in this study shows more sensitive in detecting acute kidney damage compared to serum creatinine.