The influence of the mode of enzyme preparation on enzymatic enantioselectivity in organic solvents and its temperature dependence

Abstract

This study addresses the question of whether the way in which a sample of a given enzyme is prepared also influences the stereochemistry of asymmetric reactions catalyzed by enzymes inorganic solvents. It is observed that not only the enzymatic enantioselectivity but also its temperature dependence varies greatly with the mode of enzyme preparation. The enantioselectivities of subtilisin Carlsberg and Rhizomucor meibei lipase in organic solvents are specifically investigated.