Actinoplanes bogoriensis sp. nov., a novel actinomycete isolated from leaf litter

Abstract

A novel actinomycete, designated LIPI11-2-Ac043 T, was isolated from leaf litter collected in Indonesia. According to phylogenetic analysis based on the 16S rRNA gene sequence comparisons, strain LIPI11-2-Ac043 T was closely related to Actinoplanes abujensis A4029 T (99.3%) and Actinoplanes brasiliensis DSM 43805 T (98.8%). Spores of strain LIPI11-2-Ac043 T were motile and the sporangia were spherical. The predominant menaquinone was MK-9(H 6) and the principal polar lipids were phosphatidylinositol, phosphatidylethanolamine, phosphatidylglycerol and diphospatidylglycerol. The whole-cell sugars were galactose, glucose and mannose; rhibose, arabinose and xylose were also detected as minor components. The major fatty acids were anteiso-C 15:0, iso-C 16:0, iso-C 15:0 and anteiso-C 17:0. These data supported the affiliation of strain LIPI11-2-Ac043 T to the genus Actinoplanes. Meanwhile, the results of DNA-DNA hybridization and physiological and biochemical tests indicated that strain LIPI11-2-Ac043 T can be distinguished from its closest related species. Therefore, strain LIPI11-2-Ac043 T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes bogoriensis sp. nov. is proposed. The type strain is LIPI11-2-Ac043 T (=InaCC A522 T =NBRC 110975 T).