Response of Bali cows on superovulation for in-vivo embryo production

Abstract

The aim of this study was to know the response of Bali cows on superovulation for embryo production. A total of two Bali cows (BC1 and BC2) obtained and selected from smallholder farms were used in the present study. These cows were selected based on good general health, body condition score, and reproductive organ. The cow that absent of health disorder and body injury as well as had a body condition score of 6 or 7 were subsequently subjected for reproductive organ examination. The cow that did not have any reproductive organ disorder was then examined using ultrasound for ovaries. Those cows that have a number of follicles at the ovary(es) with or without corpus luteum were selected as donors. The cows were superovulated using hormones and inseminated artificially at a fixed time. Embryos were flushed at day-seven after insemination. Parameters measured were 1) response of Bali cows on superovulation treatment; 2) the number of embryos at each ovary; 3) the quality of embryos, and 4) embryo development stage. The results of this study showed that Bali cows were responded to superovulation. After first superovulation, BC1 produced five follicles; one in the left ovary and four in the right ovary. On the other hand, BC2 produced seven follicles; two in the left ovary and five in the right ovary. It can be concluded that Bali cows responded to superovulation.