DEVELOPMENT OF AN INFANT FORMULA HIGH IN ALPHA-LACTALBUMIN WITH ONLY A2 BETA-CASEIN BY SPRAY DRYING, DESIGNED TO RESEMBLE THE PROTEIN COMPOSITION OF HUMAN MILK

Abstract

In thiswork, an infant formula enriched in α-lactalbumin and whose β-casein fraction contains onlyA2 β-caseinwas development González y M. Gutiérrez-Rojas by spray drying. Experimental infant formula (EF) was designed to closely resemble the protein composition of human milk. 259 Crecimiento, sobrevivencia y adaptación de Bifidobacterium infantis a condiciones ácidas Response surface method (RSM) with two factors: total solids and inlet air temperaturewas used for the optimization of the spray drying process. Response variables were solubility, moisture content, luminance and peroxide value. To define optimal drying (Growth, survival and adaptation of Bifidobacterium infantis to acidic conditions) conditions, a stability test was performed for two treatments (120 °C, 40 wt. % and 170 °C, 30 wt. %), which were obtained L. Mayorga-Reyes, P. Bustamante-Camilo, A. Gutiérrez-Nava, E. Barranco-Florido y A. Azaola- from the RSM. The treatment with 170 °C and 30 wt. % concentration of total solids was selected as the best alternative for the Espinosa spray drying process. The protein composition in the EF was 37.1% casein, 23.1% α-lactalbumin and 21.3% β-lactoglobulin. A 265 Statistical approach to optimization of ethanol fermentation by Saccharomyces cerevisiae in the decrease of 6% in β-lactoglobulin increased the relative proportion of α-lactalbumin, which favoured the similarity of the EF to breast milk. The homology of the β-caseins with the A2 β-casein isoform was 99.82%. presence of Valfor® zeolite NaA