An acyl-SAM analog as an affinity ligand for identifying quorum sensing signal synthases

Abstract

N-Acylhomoserine lactones (AHLs) are quorum sensing signals produced by Gram-negative bacteria. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM. © The Partner Organisations 2014.