Nonomuraea indica sp. Nov., novel actinomycetes isolated from lime-stone open pit mine, India

Abstract

A Gram-positive, aerobic, nonmotile actinomycete strain designated DRQ-2 T was isolated from the soil sample collected from lime-stone open pit mine from the Gulbarga region, Karnataka province, India. Strain DRQ-2 T was identified as a member of the genus Nonomuraea by a polyphasic approach. Strain DRQ-2 T could be differentiated from other members of the genus Nonomuraea on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain DRQ-2 T showed highest sequence similarity to Nonomuraea muscovyensis DSM 45913 T (99.1%), N. salmonea DSM 43678 T (98.2%) and N. maheshkhaliensis JCM 13929 T with 98.0%, respectively. Chemotaxonomic properties showing predominant menaquinones of MK-9 (H 4), MK-9(H 2) and MK-9(H 6), major polar lipids comprised diphosphatidylglycerol, phosphatidylmono methyl ethanolamine (PME), phosphatidylethanolamine (PE), hydroxy-PME (OH-PME), hydroxy PE (OH-PEE), phosphatidylglycerol (PG), ninhydrin-positive phosphoglycolipid and unknown phospholipid, fatty acids with major amounts of i-C 16:0, ai-C 15:0 and ai-C 17:0 supported allocation of the strain to the genus Nonomuraea. Results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of strain DRQ-2 T from closely related species. The genomic DNA G+C content of the organism was 72.5 mol%. On the basis of phenotypic, chemotypic and molecular characteristics, strain DRQ-2 T represents a novel species of the genus Nonomuraea, for which the name N. indica sp. Nov. is proposed, with type strain DRQ-2 T (=NCIM 5480 T = CCTCC AA 209050 T).